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KMID : 1134820110400010078
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Journal of the Korean Society of Food Science and Nutrition
2011 Volume.40 No. 1 p.78 ~ p.83
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Antihepatotoxic and Antigenotoxic Effects of Herb Tea Composed of Chrysanthemum morifolium Ramat
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Lee Hyun-Jung
Hwang Young-Il
Park Eun-Ju
Choi Sun-Uk
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Abstract
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The flower of Chrysanthemum morifolium Ramat. with antioxidant, anticancer, and anti-inflammatory functions has been a widely used traditional herb as a healthy beverage and medicine. The aim of the present study was to investigate a herb tea consisting of C. morifolium Ramat., Corni fructus and Schizandra chinensis Baillon for its hepatoprotective activity against CCl₄-induced toxicity in freshly isolated rat hepatocytes and antigenotoxic effect against oxidative stress induced DNA damage in human leukocytes. Three different compositions of the herb tea (Mix Ⅰ, Ⅱ, and Ⅲ) were prepared by extracting with water at 90℃. Freshly isolated rat hepatocytes were exposed to CCl₄ along with/without various concentrations of each tea. Protection of rat primary cells against CCl₄-induced damage was determined by the MTT assay. The significant antihepatotoxic effect of the tea was shown in Mix Ⅰ and Ⅱ. The increased transaminase (AST and/or ALT) release in media of CCl₄ treated hepatocytes was significantly lowered by all the teas tested. The effect of the tea on DNA damage in human leukocytes was evaluated by Comet assay. All teas showed a protective effect against H₂O₂-induced DNA damage. From these results, it is assumed that herb tea based on C. morifolium Ramat., Corni fructus and Schizandra chinensis Baillon exerted antihepatotoxic and antigenotoxic effects.
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KEYWORD
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Chrysanthemum morifoliumRamat, , Corni fructus, Schizandra chinensisBaillon, antihepatotoxity, antigenotoxictiy
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